Inflammatory Bowel Disease Models
DSS & TNBS IBD Rodent Models
Human Inflammatory Bowel Disease (IBD) encompassing two main illnesses, Crohn’s disease and Ulcerative Colitis, are chronic wasting conditions of unknown etiology for which there is still no cure. They are characterized clinically by diarrhea and body weight loss. Histopathogical signs include leukocyte infiltration, pronounced epithelial hyperplasia, depletion of mucin secreting goblet cells and finally ulceration.
An early event though to participate in the pathogenesis of IBD is the disruption of the gastrointestinal epithelial barrier. This disruption leads to the mixing of microbial pathogens from the lumen with antigen presenting cells in the lamina propria producing an inflammatory response. The resulting pro-inflammatory cytokines and chemokines recruit and activate leukocytes, regulate the integrity of the epithelial barrier and stimulate the production of chemokines from epithelial cells. Together these events lead to chronic inflammation in the intestines.
Rodent models of IBD are commonly used to evaluate the mechanisms of gastrointestinal inflammation. DSS and TNBS are two commonly used models for this purpose.
TNBS-Induced Model of Human Inflammatory Bowel Disease (IBD)
Model of Gastrointestinal Inflamation and Crohn's Disease
Species available: Mouse TNBS, Rat TNBS
TNBS-induced intestinal inflammation is commonly used in mice as a model for IBD, particularly in reference to Crohns disease (CD).
For our 7-day TNBS model, we induce disease through intrarectal administration of TNBS, to produce clinically amenable indicators to that of Crohn's Disease. Indicators include: diarrhea, rectal bleeding, body weight loss, more diffuse intestinal inflammation, occasional adhesions, fibrosis and vascularized ulceration. Disease indications can also be significantly reduced for a less servere phenotype via a one-time i.p. injection of anti-p40 antibody.
Dosing routes | Assessments available |
---|---|
PO | Body weight loss |
IV | Colitis score |
IP | Colon length/weight |
SC | Gross Pathology |
Infusion Pump | Biomarkers |
Histology/IHC | |
PK |
Colonic Sections from TNBS-Induced IBD Model in Mice Processed via "Swiss Roll" Technique to Preserve Morphology (Periodic Acid Schiff/PAS Staining):
DSS-Induced of Human Inflammatory Bowel Disease (IBD)
Species available: Mouse Acute DSS, Mouse Chronic DSS, Rat Acute DSS
Our DSS model is a chemically-induced study of Inflammatory Bowel Disease (IBD) using dextran sodium sulphate (DSS) in drinking water that induces ulcerative colitis-like inflammation. And the disease is descending colon specific characterized by; gross bleeding, thickened colon walls with occasional adhesions, pseudo polyps, redness and edema, as well as reduced overall colon length.
- For Acute Colitis models - we induce a continuous administration of DSS over the course of 5 days.
- For Chronic Colitis models - we induce by administering DSS in sterile drinking water in alternating cycles.
Our model is extremely useful for studying the role of innate leukocyte cells such as macrophages and neutrophils in induction of disease, because the pathology of the DSS IBD model involves general innate inflammation. In addition, a one-time i.p. injection of anti-p40 anitbody significantly reduces disease severity.
Dosing routes | Assessments available |
---|---|
PO | Body weight loss |
IV | Colitis score |
IP | Colon length/weight |
SC | Gross pathology |
Infusion pump | Biomarkers |
Cannula | Histology/IHC |
PK |