Cell Culture Modeling
In Vivo & In Vitro Assays
Evaluate the Immunosuppression or Immunogenicity Properties of Compounds
ImmuneProfiler is a suite of assays that can be customized to determine a compounds properties on the immune response. We offer a variety of in vivo models and in vitro assays for evaluating select pathways. Sponsors can select pathways of interest, choose between stimulation or inhibition assays, evaluate in cell based or whole animal systems.
Readouts include:
- Specific cytokine panels, FACs analysis
- Investigation of Th1 Th2, Th17, CD8, B-cell response in vivo
- In vitro methodologies and efficacy testing evaluating macrophage and T-cell inhibition and stimulation, proliferation and cytokine release
The tables below outline the various models and read-outs.
In Vivo Immune Response Models:
Category | Species | Response | Output |
Cellular Immunity | mouse | Th1 response | cytokine panel, FACS |
Cellular Immunity | mouse | Th2 response | cytokine panel, FACS |
Cellular Immunity | mouse | Th17 response | cytokine panel, FACS |
Cellular Immunity | mouse | CD8 T cell response | cytokine panel, FACS |
Non-specific Immunity | mouse | innate | cytokine panel, FACS |
Humoral Immunity | mouse | B cell response | FACS, cytokines |
Humoral Immunity | mouse | B cell activation | FACS |
Allergy/Hypersensitivity | mouse/rat | Th1 | cytokines, FACS |
Allergy/Hypersensitivity | mouse | Th2 | cytokines, FACS |
In Vitro Immune Response Assays
Model | System | Stimulant(s) | Output |
---|---|---|---|
Custom Model | Custom | Custom | Cell proliferation, multiplex cytokine panel, ELISA, qPCR, FACS |
PBMC Cellular Inflammation Model | Periperipheral blood mononuclear cells (PBMC) | Lipopolysaccharides (LPS), Sponsor Compound | Cell proliferation, multiplex cytokine panel, ELISA, qPCR, FACS |
T Cell Activation | PBMC | antiCD3/CD28, ConA, PHA, Sponsor Compound | Cell proliferation, multiplex cytokine panel, ELISA, qPCR |
In vitro B-cell stimulation/activation | PBMC or B-cells | anti-CD40, anti-IgM/IgG, IL-4, Sponsor Compound | Cell proliferation, multiplex cytokine panel, ELISA, qPCR, FACS |
M0/M1/M2 Macrophage Polarization Assay | Monocyte cell line THP-1 | phorbol 12-myristate-13-acetate (PMA), LPS, IFN-gamma, IL-4, IL-13,Sponsor Compound | Cell proliferation, multiplex cytokine panel, ELISA, qPCR |
TH17 Cell Differentation | CD4+ T cells | MACSiBeads (loaded with CD2, CD3, CD28 antibodies), Th-17 differentiation cocktail containing TH17 poloarizing cytokines (IL-1b, IL-6, IL-23, TGF-B1, anti-IFNg, anti-IL-4), Sponsor Compound | Cell proliferation, multiplex cytokine panel, ELISA, qPCR, FACS |
Fibroblast-like Synoviocyte Inflammation Assay | Healthy Human Fibroblast-like Synoviocytes (HFLS) or Rheumatoid Arthritis Patient (HFLS-RA) | TNF-a, Sponsor Compound | Cell proliferation, multiplex cytokine panel, ELISA, qPCR |
Colonic Epithelial Cell Inflammation Assay | Colonic Epithelial Cells | TNF-a, IL17a, Sponsor Compound | Cell proliferation, multiplex cytokine panel, ELISA, qPCR |